This study aimed to assess nutritional status and risk factors for chronic non-transmissible diseases in 1,252 factory employees in Santa Catarina State, Brazil. The following variables were studied: nutritional status, waist circumference, blood pressure, blood glucose levels, age, and schooling. Overweight and obesity were observed in 45% and 10% of the sample, respectively, with a significant association between nutritional status and gender, but not between nutritional status and schooling, adjusted for age. The proportion of high waist circumference was 33%, with a significant difference between genders. High systolic blood pressure (SBP) and diastolic blood pressure (DBP) were present in 18% and 11% of the employees, respectively, with a significant association between genders after adjusting for age. Type II diabetes mellitus was diagnosed in 2% of the sample and altered glucose levels in 4%. After adjusting for age, the only variables significantly associated with waist circumference were SBP and DPB. The results allowed the implementation of preventive and educational activities to improve employees' quality of life and productivity.